Biospecimen Repository and Processing Lab, Canada

Certification Status: Approved

Registration Status: Completed

Objective: The Ontario Familial Breast Cancer Registry, Ontario Cancer Genetic Network, Sarcoma Tissue Bank, Ontario Familial Colon Cancer Registry, Familial Gastrointestinal Registry, the Ontario Pancreas Cancer Study, the Ontario Health Study and the Ontario Birth Study are the major studies for which biospecimens are processed and stored. The Biospecimen Repository and Processing Lab provides services to process biospecimens such as blood, saliva, urine, tumour tissue (fresh frozen or paraffin embedded tissue) as well as isolate DNA or RNA from these source samples. The fully automated robotic aliquoting system (Tecan freedom evo) is used for aliquoting biospecimens for large scale studies. Manual aliquoting and processing systems are used for smaller studies which allows for customization. The robotic DNA extraction system (Tecan freedom evo) utilizes Promegaâ€™s Reliaprep chemistry to extract DNA from 3 to 10 milliliters of blood or 5 milliliters of saliva samples. This automated system incorporates DNA quantiation by both spectrophotometry and fluorometry. Manual extraction protocols are available when smaller DNA yields, such as paraffin embedded tissue, is expected. Upon request, biospecimens are assembled and provided to researchers with proper approvals.

Registered Biobank Name	Biospecimen Repository and Processing Lab
Biobank Leader	Abiola Oduwole
Country	Canada
Email for biobank inquiries	oduwole@lunenfeld.ca
Principal Investigator	Abiola Oduwole
Website	Dr. Irene Andrulis, Dr. M. Toone
User Type Mono: A biobank that supports a specific research project, may have few staff members, a small-scale accrual scope with little to no initial intention of releasing or distributing biospecimens to secondary parties Oligo: A biobank that supports several research groups or clinical trials, may or may not be designed to release biospecimens outside their collaborative group Poly: A biobank that has generally a larger accrual scope, resources, and multiple users outside the biobank proper	Oligo - Collection aimed at supporting several research projects, a research group or a research consortium

Biospecimen Collected:

Cohort Highlights

100 cases Next Generation Sequencing assay to detect all classes of genetic variant in Lung Cancer using a single Diagnostic test.
200 cases Colorectal TMA for QC purposes in support of study of tumor infiltrating lymphocytes in ovarian cancer tissues.
Build 300 cases breast cancer TMA containing primary tumour and matched metastatic LN for various subtypes (luminal, HER2, Triple negative). Start with Triple negatives.
125 cases Breast tumour and metastatic lymph nodes in breast cancer cases with outcomes of metastasis to lung or bone.
100 cases of rare tumours - fibrolamellar hepatocellular carcinoma. FFPE, frozen, BC.
200 cases of FFPE tissue sections from breast, lung adenocarcinoma, and lung squamous cell carcinoma with matching normal tissue (only pathology data requested).